update 17/01/2021, online 20/01/2001
rotation in a
- now hold
by a fast
DIY progress @ 2020 :
getting a single FRET-labeled FoF1-ATP synthase into an
Anti-Brownian electrokinetic trap (ABELtrap) and recording
fast subunit rotation during ATP hydrolysis at 100µM [ATP].
Labeling positions yield two distinct FRET levels ‘L’ and ‘H’.
Single-molecule catalysis results in fluctuating FRET levels.